Fluorescent Cell-Counting Neutralization Test for Psittacosis

Quantitative Assay of Psittacosis Virus by the Fluorescent Cell-counting Technique.

The studies described here indicate that the fluorescent cellcounting technique provides a rapid, precise, reproducible, and sensitive procedure for the assay of infective particles of psittacosis virus. The efficiency of virus adsorption onto coveralip cultures of McCoy cells was markedly increased by the use of centrifugal force; assay values were 1.0 log higher than those obtained after stat...

Fluorescent dot counting in interphase cell nuclei

Fluorescence in situ hybridization allows the enumeration of chromosomal abnormalities in interphase cell nuclei. This process is called dot counting. To estimate the distribution of chromosomes per cell, a large number of cells have to be analysed, particularly when the frequency of aberrant cells is low. Automation of dot counting is desirable because manual counting is tedious, fatiguing, an...

Comparison of Micro Neutralization Test with Hemadsorption-Inhibition Test, and Immunoflrorescence Technique for Detecting Mumps Antibodies

Comparison of a Micro-Neutralization Test with the Rapid Fluorescent Focus Inhibition Test for Measuring Rabies Virus Neutralizing Antibodies.

The rapid fluorescent focus inhibition test (RFFIT) is routinely used in the United States to measure rabies virus neutralizing antibodies (rVNA). RFFIT has a long history of reproducible and reliable results. The test has been modified over the years to use smaller volumes of reagents and samples, but requires a 50 μL minimum volume of test serum. To conduct pathogenesis studies, small laborat...

Automation of Fluorescent Dot Counting in Cell Nuclei

We have developed a completely automated fluorescence microscope system that can examine 500 cells in approximately 20 minutes to determine the number of labeled chromosomes (seen as dots) in each cell nucleus. This system works with two fluorescent dyes – one for the DNA hybridization dots (e.g. FITC) and one for the cell nucleus (e.g. DAPI). After the stage has moved to a new field the image ...